Correct setup of the substantia nigra requires Reelin-mediated fast, laterally-directed migration of dopaminergic neurons

Correct setup of the substantia nigra requires Reelin-mediated fast, laterally-directed migration of dopaminergic neurons

Blog Article

Midbrain dopaminergic (mDA) neurons migrate to form the laterally-located substantia nigra pars compacta (SN) and medially-located ventral tegmental area (VTA), but little is known about the underlying cellular and molecular processes.Here we visualize the dynamic cell morphologies of tangentially migrating SN-mDA neurons in 3D and identify two distinct migration modes.Slow migration is the default mode in SN-mDA neurons, while fast, laterally-directed migration occurs infrequently and Modern Miscellaneous is strongly associated with bipolar cell morphology.Tangential migration of SN-mDA neurons is altered in BotBots absence of Reelin signaling, but it is unclear whether Reelin acts directly on migrating SN-mDA neurons and how it affects their cell morphology and migratory behavior.

By specifically inactivating Reelin signaling in mDA neurons we demonstrate its direct role in SN-mDA tangential migration.Reelin promotes laterally-biased movements in mDA neurons during their slow migration mode, stabilizes leading process morphology and increases the probability of fast, laterally-directed migration.